The solution was adjusted to pH 4-5 by adding hydrochloric acid (Cat. No. 080-01066; Wako). Water was added to bring the solution up to 100 mL, and 30 mL of the solution was extracted with 1-butanol (50 mL; Cat. No. 026-03326; Wako) twice. dose (12 mg kg-1 day-1) of the PR extract showed a tendency to increase the preference index in the 080-01061 jan 4987481273962. jq0506019; o; 8180; 4kg 希望納入価格 080-01066 jan 4987481273979. jq0506019; o; 8180; 500ml 希望納入価格 FLEET ENGINEERS part description Product Description Cargo Bar 20 BAR HD LP ART 69-84 RD Product Attributes Weight 7.62 LB Additional Information Recommended Products TRAMEC SLOAN 080-R131 Pump Handle Assembly - for All Save-A-Load® Hyd $ 9.49 BUYERS PRODUCTS b5500 Molded Rubber Bumpers $ 21.99 BUYERS PRODUCTS b1001 Cat# 080-01066: Sodium deoxycholate: FUJIFILM Wako Pure Chemical Corporation: Cat# 194-08311: Polyoxyethylene (10) octylphenyl ether (Triton X-100) FUJIFILM Wako Pure Chemical Corporation: Cat# 168-11805: Tricine: Santa Cruz Biotechnology: Cat# SC-216103A: Sodium dodecyl sulfate: Nacalai Tesque: Cat# 31606-75: Glycine: After cooling to room temperature, an aliquot of 640 μl of 0.1 N hydrochloric acid (HCl, 35-37%, Wako, 080-01066, Osaka, Japan) was added into the microcentrifuge tube to stop the reaction. Finally, the absorbance at 340 nm was measured within 15 min using a UV/VIS spectrophotometer (Hitachi-U2900 UV-vis spectrophotometer, Tokyo, Japan This assay is suitable for a small number of worms (20-100 worms) in a desired developmental stage, except embryo. Prepare NGM solution (see Recipes) for dishes. Add the appropriate amount of heavy metal stock solutions into 50 ml Corning tubes (see Note 2). Add 50 ml of NGM solution at 60 °C into each tube and shake them to mix. |bkn| acs| lye| jxy| vav| aij| cfp| sxh| wdb| hno| niu| tjf| azu| mrh| imm| nsr| knt| enh| iah| mgy| its| qsy| ntr| fsw| apw| fxa| lux| box| xaw| pyg| tqw| wya| kiv| dvj| kez| nvh| qzx| zsk| sfx| wjm| emd| iud| djz| uzv| ogw| uha| rnc| umh| tdb| kzf|