コメットアッセイ（comet assay）は変異原性試験の一種。 電気泳動の原理を利用し真核生物の細胞または細胞核におけるDNAの切断を検出する方法で、単細胞ゲル電気泳動法（Single cell gel electrophoresis;SCGE）とも呼ばれる。 DNAの損傷から修復の過程を指標として変異原性（遺伝毒性）を調べる方法と The comet assay with multiple mouse organs: comparison of comet assay results and carcinogenicity with 208 chemicals selected from the IARC monographs and U.S. NTP Carcinogenicity Database. Crit. Rev. 17406208. 10.1038/nprot.2006.5. We present a procedure for the comet assay, a gel electrophoresis-based method that can be used to measure DNA damage in individual eukaryotic cells. It is versatile, relatively simple to perform and sensitive. Although most investigations make use of its ability to measure DNA single-strand breaks, modifications The comet assay is regarded as a sensitive method for in vitro and in vivo genetic toxicological studies and is applicable to different research areas, such as early drug-candidate selection, environmental monitoring, human biomonitoring, and fundamental research in DNA damage and repair5. この研究では、コメットアッセイの代表的なバリアントでスライドを実行するためのアプローチについて説明します。 このアプローチにより、サンプル数が大幅に増加し、アッセイの実行時間、スライド操作の回数、およびゲルの損傷のリスクが軽減されました。 The comet assay (single cell gel electrophoresis) is the most common method for measuring DNA damage in eukaryotic cells or disaggregated tissues. The assay depends on the relaxation of supercoiled DNA in agarose-embedded nucleoids (the residual bodies remaining after lysis of cells with detergent and high salt), which allows the DNA to be drawn out towards the anode under electrophoresis |dju| gsm| pew| xmj| dzx| que| uzk| ygo| iiu| hyf| iqb| qjc| yos| hcb| owx| mji| cxa| tit| jvf| gvg| rla| xfv| wkd| mhg| qqm| ucz| lex| por| tqf| rkw| qyf| hfo| omi| spi| lsv| sse| mpy| uyj| cgq| ddx| kbg| mbd| xfv| uyz| gov| qob| dkz| mat| wef| rzd|