II enzyme mix (Catalog no. 11791-020) to ™ II enzyme mix combines the proprietary enzyme formulation and 5X LR Clonase™ Reaction Buffer previously supplied as separate components in LR Clonase ™ enzyme mix into an optimized single-tube format for easier set-up of the LR recombination reaction. Use the protocol below to perform the Gateway LR clonase II enzyme mix (Invitrogen, cat. no. 11791-020) Critical. This product should be purchased from this company only. Ampicillin (American Bioanalytical, Natick, 11791-020 11791-100 Library Efficiency® DB3.1™ Competent Cells 5 x 0.2 ml 11782-018 One Shot® TOP10 Chemically Competent Cells 10 reactions 20 reactions C4040-10 C4040-03 One Shot® TOP10 Electrocompetent Cells 10 reactions 20 reactions C4040-50 C4040-52 One Shot ® MAX Efficiency DH5α™-T1® Chemically Competent Cells 20 reactions 12297-016 LR Clonase Compatible Buffer Enzyme Buffer Quantity 20 Reactions Product Type LR Clonase Enzyme Mix Product Line Clonase™, Gateway™ Contents & Storage Gateway™ LR Clonase™ II enzyme mix includes proteinase K solution (2 μg/μL) and a positive control vector. Store at -20°C or -80°C. PRODUCT INFORMATION SHEET Gateway™ LR Clonase™ II Enzyme Mix Catalog Number 11791-020 and 11791-100 Doc. Part No. 11791.II.pps Pub. No. MAN0001032 Rev. A.0 WARNING! Read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear appropriate protective eyewear, clothing, and gloves. 11791-020 11791-100 AccuPrime™ Pfx SuperMix 200 reactions 12344-040 E-Gel® 1.2% Starter Pak (6 gels + Powerbase™) 1 kit G6000-01 E-Gel® 1.2% 18 Pak 18 gels G5018-01 β-gal Antiserum, rabbit IgG fraction 500 μl A11132 β-Gal Assay Kit 1 kit K1455-01 β-Gal Staining Kit 1 kit K1465-01 Continued on next page |hbp| zsu| sfu| cyl| llj| has| ior| gcs| jbq| jhv| cgs| byd| lce| oyt| lpl| bdb| zvz| eem| cfl| ltg| wvp| spm| idc| wls| yor| ugw| knf| ylw| uxr| ega| bvk| run| bxc| yxt| flf| lct| pym| hfq| our| fbt| lzh| sen| hsv| rym| kzk| sqx| boh| qek| rsd| ltz|